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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Reprod Sci. 2010 Aug 16;17(10):955–962. doi: 10.1177/1933719110376092

Figure 2.

Figure 2

Cortisol production in FACS from control and LTH ovine fetuses as described in the Methods section. A) untreated basal, treated with Diethyleneamine (DETA-NO 0.5 nM), or a combination of L-Arg (2mM) and L-NAME (1mM). B) treated with ACTH (10nM) alone or pre-treated with Diethyleneamine (DETA-NO 0.5 nM), or a combination of L-arginine (2mM) and L-NAME (1mM) followed by ACTH treatment. C) Cortisol data normalized to % change from ACTH treatment alone. (n=4 for control and 6 for LTH). LTH indicates long-term hypoxia; FACs, fetal adrenal cortical cells; L-Arg, L-arginine; L-NAME, nitro-L-arginine methyl ester; ACTH, adrenocorticotropic hormone.