Figure 7. Deregulated surface adhesion of T cells lacking MyoIIA.

a) Representative adhesion areas of control and blebbistatin treated CD8⁺ activated T cells plated on glass coated with casein or ICAM-1. Overlays of the adhesion areas measured by TIRF microscopy (green) and of the brightfield channel are shown for each condition. White scale bars represent 10 µm. b) Quantification of the adhesion areas (±SEM) of control and blebbistatin-treated CD8⁺ activated T cells on casein (left) or ICAM-1 (right). c) Quantification of the adhesion areas (±SEM) of control and MyoIIA cKO naïve CD8⁺ T cells plated on ICAM-1. d) The percentage of transferred control and MyoIIA cKO naïve T cells in contact with the fibroblastic reticular cell (FRC) network in actin promoter-CFP chimeric mice was quantified by 2-photon microscopy. The average (±SEM) from multiple stage positions imaged from three independent experiments is shown. e) CFSE-labeled naïve CD8⁺ T cells from control or MyoIIA cKO mice were mixed with CMTMR-labeled FRCs, lymphatic endothelial cells (LECs), dendritic cells (DCs), or naïve T cells as indicated. The average conjugation percentage (±SEM) of control and MyoIIA cKO T cells to each indicated cell type after 10 min incubation was quantified by FACS. Data from a–c are representative or pooled from at least two independent experiments; data from d and e are pooled from at least three independent experiments.