Skip to main content
. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Plasmid. 2010 Jun 25;64(3):150–155. doi: 10.1016/j.plasmid.2010.06.003

Figure 4.

Figure 4

Figure 4

A. PCR amplification of tet(M) region of 12 transconjugants resulting from a mating between E. faecium C68 and E. faecium TX1330. Lane 1 is the recipient strains (negative control). LANES 2–13: Transconjugants CV621-632. Lane 14: E. faecium C68 grown without tetracycline; Lane 15: C68 grown with tetracycline; Lane 16: 1 kb size standard. Sizes are marked to the right of the figure. The arrows indicate the additional band resulting from amplifying this segment in Tn6084, which has the insertion of ISEfa11 in the region. B. PCR amplification using primers ACJQ_149_7990 and 6773_B designed to amplify a segment from within ISEfa11 to a flanking region. The results confirm that transconjugant CV630 has a copy of Tn6084 while transoconjugants CV631 and CV632 do not.