Fig. 3.

Effects of triiodothyronine (T3) exposure on the expression of thyroid hormone-related genes in Silurana tropicalis premetamorphic tadpoles. Silurana tropicalis (Nieuwkoop and Faber stage 52–54) were exposed to T3 (0, 0.5, 5, 50 nm) for 48 h. Effects of T3 on tralpha and trbeta (a), dio1, dio2 and dio3 (b) and the reference genes rpl8, gapdh and 18S (c) are presented. Data are presented as the fold changes relative to control and are normalised to RNA content (note the broken y-axis for a and b). Bars represent the mean ± SEM. Asterisks represent significant differences from the control group (n = 8 pools; *P<0.05; **P<0.01; ***P<0.001). Note that the scales of the y-axis vary between genes.