Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 May 10;38(17):5774–5783. doi: 10.1093/nar/gkq336

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Gel filtration analysis of the archaeal GatDE:GluRS binary complex. Gel filtrations over a sephacryl S300 16/60 column were carried out as described in the ‘Materials and Methods’ section with GatDE (10 µM) and/or ND-GluRS (20 µM) added to the mix. Gel filtration analysis of (A) GatDE alone, (B) ND-GluRS alone and (C) GatDE and ND-GluRS pre-incubated together. (D) Combined aminoacylation/amidotransferase assay with ³²P-labeled tRNA^Gln (100 nM), ATP (4 mM), l-Glu (2 mM) and Asn (2 mM) incubated with (lane 1) no enzyme, (lane 2) purified GatDE (250 nM), (Lane 3) purified ND-GluRS (250 nM), (Lane 4) purified ND-GluRS and GatDE (250 nM each) or (lane 5) the ND-GluRS:GatDE complex (400 nM) eluted from the gel filtration column (Figure 2C). (E) Calibration curve with known molecular weight standards (filled circle) and the ND-GluRS:GatDE complex (open circle).