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. 2010 May 10;38(17):5774–5783. doi: 10.1093/nar/gkq336

Figure 3.

Figure 3.

Formation of the ND-GluRS:GatDE:tRNAGln complex. Electrophoretic mobility shift assays were performed between purified ND-GluRS and GatDE with 32P-labeled tRNAGln or tRNAGlu as described in the ‘Materials and Methods’ section. (A) 32P-labeled tRNAGln (8 µM) incubated with no enzyme (lane 1) or increasing concentrations of 32P-labeled tRNAGln incubated with GatDE (1 µM; lanes 2–8). (B) 32P-labeled tRNAGln (100 nM) incubated with (lane 1) no enzyme, (lane 2) GatDE (6.0 µM), (lane 3) ND-GluRS (1 µM) or (lanes 4–9) ND-GluRS (1 µM) and GatDE (1.0–6.0 µM). (C) 32P-labeled tRNAGlu (100 nM) incubated with (lane 1) no enzyme, (lane 2) ND-GluRS (1 µM), (lane 3) GatDE (6 µM) or (lanes 4–7) ND-GluRS (1 µM) and GatDE (1.0–6.0 µM).