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. 2010 May 10;38(17):5774–5783. doi: 10.1093/nar/gkq336

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Gel filtration analysis of the archaeal GatDE:GluRS:tRNA^Gln complex. Gel filtrations over a sephacryl S300 16/60 column were carried out as described in the ‘Materials and Methods’ section with tRNA^Gln (20 µM) and protein(s) indicated (GatDE and/or ND-GluRS) (20 µM). (A) Gel filtration and native PAGE analysis of free tRNA^Gln. (B) Gel filtration and native PAGE analysis of a sample containing GatDE with tRNA^Gln. The arrow indicates how long approximately GatDE is retained on the column (Figure 2A). (C) Gel filtration and native PAGE analysis of a sample containing GluRS and tRNA^Gln. (D) Gel filtration and native PAGE analysis of a sample containing GluRS, GatDE and tRNA^Gln.