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Gel filtration analysis after aminoacylation and transamidation. Gel filtrations over a sephacryl S300 16/60 column were carried out as described in the ‘Materials and Methods’ section with tRNAGln, GatDE and ND-GluRS, (20 µM each) and either (A) ATP (4 mM) and l-Glu (3.5 mM), or (B) ATP (4 mM), l-Glu (3.5 mM) and Asn (4 mM) added to the incubation mixture.
