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Transcription activity of RNAPs with substitutions of β′R339. Transcription was performed either in the absence (lanes 1–4) or in the presence (lanes 5–8) of the CpA primer (100 µM) as described in ‘Materials and Methods’ section. The position of the 53 nt run-off RNA (RO) is indicated by an arrowhead. The ratio of activity of each mutant RNAP to that of the wild-type enzyme is shown below the gel.
