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Stability of promoter complexes formed by RNAPs with substitutions of β′ R339. (A) Stability of the T7A1 promoter complexes. Promoter complexes were incubated in the presence of 10 µg/ml heparin for time intervals varying from 10 s to 4 min, and the remaining activity was measured in the reaction of trinucleotide synthesis. (B) Stability of the λPR promoter complexes. The incubation time was varied from 30 s to 120 min. Promoter complex half-lives are indicated below each gel panel.
