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. 2010 May 10;38(17):5784–5796. doi: 10.1093/nar/gkq355

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Stability of elongation complexes formed by RNAPs with substitutions of β′ R339. Halted A26 elongation complexes were formed at the λP_R promoter and incubated in the presence of 1M NaCl. The RNA transcripts that remained bound to RNAP after different time intervals were analyzed by gel electrophoresis. The 26-mer RNA transcript is shown by an arrowhead. Mutant RNAPs also synthesized considerable amounts of a shorter RNA transcript, likely as a result of defects in promoter escape and/or RNA elongation.