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The effect of the R339 substitutions on cell viability. The growth of the RL602 cells containing a temperature sensitive rpoC allele was assayed at either permissive (32°C, upper row) or restrictive (42°C, lower row) temperature as described in ‘Materials and Methods’ section. The cells expressed either wild-type (R) or mutant β′ subunits (K, E and A) from corresponding plasmids, or contained a control pBR322 plasmid (‘−’).
