Figure 4.

Expression analysis of cis-encoded antisense npcRNA candidates at different growth stages of S. typhi and E. coli. Northern blot analyses were performed on total RNA isolated from different growth stages of S. typhi and E. coli at different OD₆₀₀ values, as shown at the top. OD₆₀₀ 0.3: lag phase-steady bacterial growth; OD₆₀₀ 0.7: log phase-exponential bacterial growth; and OD₆₀₀ 1.5: stationary growth phase. The npcRNA candidates and RNA lengths are indicated. As loading controls, a few examples of ethidium bromide-stained 5S rRNA are shown.