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. 2010 May 7;38(17):5851–5866. doi: 10.1093/nar/gkq292

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Time course of the interactions between SibC and ibsC RNA. (A) ³²P-labeled ibsC RNA (10 nM) was incubated with 50 nM of unlabeled SibC RNA at 37°C for 1, 30, 90 and 360 s. Samples were withdrawn from each time point and digested with RNase V1 for 15 min. The reaction was stopped by ethanol precipitation. Lanes C and OH correspond to untreated ibsC RNA and alkaline hydrolysis ladders, respectively. Lane T1 corresponds to G ladders generated by RNase T1 under denaturing conditions. The regions showing significant changes in the intensities of bands are denoted as a to e. (B) The regions showing significant changes during the time course of incubation are also shown in the secondary structure model of ibsC mRNA.