Figure 8.

Aldosterone and dDAVP affect binding of RBPs to polysomes. Polysomes of hormone-treated mCCD cells were separated over sucrose gradients and fractionated as described in the legend of Figure 4. Proteins of each fraction were concentrated by TCA-precipitation redissolved in a volume of 100 µl buffer (25 mM Tris, 1% SDS). Proteins of all fractions (5 µl) of sucrose gradients were analysed for RBPs annexin II, AUF1, FMRP, hnRNP-A1, hnRNP-A2/B1, hnRNP-E1, HuR, nucleolin, TTP and the cytosolic proteins GAPDH and β-actin by western blotting using specific antibodies. A representative figure of three independent experiments is shown.