Figure 9.

Influence of over-expressed ARE-BPs on the expression of chimeric luciferase plasmids containing γ-ENaC UTRs. mCCD cells were transfected with pGL3p vector or chimeric variants, where original luciferase mRNA UTRs were substituted by rat γ-ENaC 3′-, 5′- and 3′-UTR or the 3′-UTR deletion variants 3′-UTRdelAU (deletion of base 2869–2958) or AU-element (base 2865–2916) of γ-ENaC mRNA. Furthermore, cells were co-transfected with expression vectors encoding for proteins of the RBPs hnRNP-A1, AUF1, FMRP, HuR or TTP. As control cells were co-transfected with empty expression vector. UTR-dependent luciferase activity was measured 24 h post-transfection. Transfection efficiency was normalized to expression of co-transfected ‘Renilla’ luciferase and relative values were normalized to the influence of empty vector control on pGL3p constructs. Data represent mean ± SD (n = 6). *P ≤ 0.05 compared to empty vector.