Figure 7.

Bisulfite sequencing of a 252-bp fragment that is located at the CAPN2 promoter region in four control and four CdLS samples. The examined fragment is located on chr1:221,966,278-221,966,529 surrounding probes cg01566170 and cg14972271 on the HumanMethylation27 array, which are 455 bp and 465 bp upstream of the TSS of CAPN2, respectively. There are nine CpG dinucleotides in this region. A solid block represents the methylated ‘C’ allele that remains as ‘C’ after bisulfite conversion and an empty block represents the unmethylated ‘C’ allele that was converted to ‘T’ after bisulfite treatment. Ten to twelve clones were selected for sequencing for each sample. Each row represents one sequenced clone and each column represents one examined ‘CpG’ site. The arrows point to probes cg01566170 and cg14972271.