. Author manuscript; available in PMC: 2010 Sep 22.

Published in final edited form as: J Am Chem Soc. 1995;117:5635–5646. doi: 10.1021/ja00126a004

Table 1.

Melting Temperatures (T_m) and Free Energies (−ΔG°₃₇) for Duplex Hairpins Bridged by Oligo(ethylene glycol) (EG) Loops and by a Natural T₄ Loop at pH 7.0

duplex hairpins	T_m^a,^b (°C)	−ΔG°₃₇^a,^b (kcal)
	62.5	3.4
	66.4	4.0
	71.1	4.8
	72.9	4.9
	74.9	3.9
	73.8	3.7
	67.8	4.6

Conditions: 2.0 μM total strand concentration, 100 mM NaCl, 10 mM MgCl₂, 10 mM Na·PIPES buffer.

Error limits for individual measurements are estimated at ±0.5 °C in T_m and ±10% in free energy. Data shown are the averages from three measurements.