Table 2.
Melting Temperatures (Tm) and Free Energies (-ΔG°37) for Triplex Hairpins Bridged by Oligo(ethylene glycol) (EG) Loops at pH 7.0
| 5′ loops | Tma,b (°C) |
−ΔG°37a,b (kcal) |
3′ loops | Tma,b (°C) |
−ΔG°37a,b (kcal) |
|---|---|---|---|---|---|
|
|
||||
| 4 | 17.7 | 3.3 | 4 | 17.8 | 5.0 |
| 5 | 20.3 | 3.6 | 5 | 19.5 | 5.0 |
| 6 | 22.1 | 4.2 | 6 | 21.5 | 5.0 |
| 7 | 23.0 | 4.4 | 7 | 22.4 | 5.2 |
| 8 | 23.5 | 4.5 | 8 | 23.3 | 5.1 |
|
|
||||
| 4 | 23.6 | 4.4 | 4 | 22.5 | 5.1 |
| 5 | 24.4 | 5.0 | 5 | 23.0 | 5.4 |
| 6 | 24.2 | 4.7 | 6 | 23.2 | 5.4 |
| 7 | 23.8 | 4.7 | 7 | 23.2 | 5.0 |
| 8 | 23.4 | 4.5 | 8 | 22.8 | 4.8 |
|
|
||||
| 6 | 23.2 | 4.4 | 6 | 22.0 | 5.3 |
a
Conditions: 2.0 μM total strand concentration, 100 mM NaCl, 10 mM MgCl2, 10 mM Na·PIPES buffer.
b
Error limits for individual measurements are estimated at ±0.5 °C in Tm and ±10% in free energy. Data shown are the averages from two to three separate measurements.