Table 3.
Melting Temperatures (Tm) and Free Energies (−ΔG°37) for Triplex Hairpins Bridged by Oligo(ethylene glycol) (EG) Loops at pH 7.0
| 5′ loops | Tma,b (°C) |
−ΔG°37a,b (kcal) |
3′ loops | Tma,b (°C) |
−ΔG°37a,b (kcal) |
|---|---|---|---|---|---|
|
|
||||
| 4 | 13.4 | 4.9 | 4 | 15.6 | 4.6 |
| 5 | 12.3 | 5.1 | 5 | 16.7 | 4.5 |
| 6 | 11.1 | 5.0 | 6 | 18.0 | 4.6 |
| 7 | 13.5 | 4.2 | 7 | 19.5 | 4.6 |
| 8 | 14.6 | 3.9 | 8 | 20.0 | 4.6 |
|
|
||||
| 4 | 15.1 | 5.5 | 4 | 23.4 | 5.5 |
| 5 | 15.0 | 5.2 | 5 | 24.0 | 5.4 |
| 6 | 16.4 | 4.8 | 6 | 24.3 | 5.6 |
| 7 | 17.8 | 4.5 | 7 | 24.4 | 5.4 |
| 8 | 18.5 | 4.1 | 8 | 23.8 | 5.8 |
|
|
||||
| 6 | 18.1 | 4.5 | 6 | 22.4 | 5.3 |
a
Conditions: 2.0 μM total strand concentration, 100 mM NaCl, 10 mM MgCl2, 10 mM Na·PIPES buffer.
b
Error limits for individual measurements are estimated at ±0.5 °C in Tm and ±10% in free energy. Data shown are the averages from 2-3 measurements.