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. Author manuscript; available in PMC: 2010 Sep 22.
Published in final edited form as: J Am Chem Soc. 1995;117:5635–5646. doi: 10.1021/ja00126a004

Table 4.

Melting Temperatures (Tm) and Free Energies (−ΔG°37) for Triple Helical Complexes Bridged by Oligo(ethylene glycol) (EG) Loops or by a Pentanucleotide Loop at pH 7.0

loop structure Tma,b
(°C)		 −ΔG°37a,b
(kcal)
graphic file with name nihms-157774-t0028.jpg
EG5 37.3 8.8
EG6 51.0 15.6
EG7 52.9 16.6
EG8 53.7 17.0
-CACAC- 52.2 14.1
graphic file with name nihms-157774-t0029.jpg
EG5 36.8 8.8
EG6 43.5 11.7
EG7 46.5 13.0
EG8 47.6 13.9
-CACAC- 52.2 14.6
a

Conditions: 2.0 μM total strand concentration, 100 mM NaCl, 10 mM MgCl2, 10 mM Na·PIPES buffer.

b

Error limits for individual measurements are estimated at ±0.5 °C in Tm and ±10% in free energy.