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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Cancer Res. 2009 Dec 22;70(1):409–417. doi: 10.1158/0008-5472.CAN-09-1353

Fig. 5.

Fig. 5

Generation and repair of DSB in BRCA2-deficient and complemented cells by LCA and/or TMZ (A) VC-8 (BRCA2-deficient) and VC-8+BRCA2 cells were incubated with 20 μM LCA, 20 μM TMZ, 20 μM LCA+20 μM TMZ, or DMSO vehicle for 3 h and fixed immediately or after 24 h recovery in untreated media for DAPI staining and γH2AX immunofluorescence. Images shown are representative of the majority of cells on coverslip. (B) The fraction of cells exhibiting >15 γH2AX foci was determined. At least 50 cells were analyzed for each treatment condition.