Figure 1.

Schematic diagram of the mechanisms for IsoAmp® HIV Assay. By using an asymmetric RT-HDA method, single-stranded DNA (ssDNA) is generated from the amplification of HIV-1 gag gene. The biotin labeled detection probe and the FITC labeled capture probe can bind to the ssDNA after the RT-HDA reaction. These probe-target hybrids then bind to streptavidin-conjugated color particles and are captured by an anti-FITC antibody striped on the DNA detection strip in the BESt™ Cassette detection.