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. Author manuscript; available in PMC: 2010 Sep 22.
Published in final edited form as: Cancer Lett. 2009 Jan 7;276(2):171–179. doi: 10.1016/j.canlet.2008.11.025

Figure 1.

Figure 1

The ubiquitous expression of S1P receptors in Wilms tumor specimens and cell lines. (A) Quantitative real-time PCR for S1P receptors mRNA expression in 10 Wilms tumor samples from COG. Expression was normalized to the expression of the housekeeping gene β-Actin. Data are the mean±SE, n=10. (B) Immunohistochemistry analysis was done to detect S1P1 protein and photos were taken under the light microscope (×20). A typical picture from nine slides is shown. (Arrow, blastemal cells; arrowhead, vascular endothelial cells). (C) Quantitative real-time PCR for S1P receptors mRNA expression in Wilms tumor cells. Expression was normalized to the expression of the housekeeping gene GAPDH. Data are the mean±SD of triplicates.