TABLE 3.
GluN2A Subunit Immunogold Labeling
| Number | ≥1 Particle | ≥2 Particles | PSD Size | |||||
|---|---|---|---|---|---|---|---|---|
| Rat | Synapses Sampled | Labeled (%) | Density (particles/PSD length) | Labeled (%) | Density (particles/PSD length) | Length (μm) | Width (μm) | Area (μm)2 |
| Control | ||||||||
| 1 | 56 | 71.4 | 12.5 | 62.5 | 19.2 | 0.197 | 0.040 | 0.008 |
| 2 | 60 | 75.0 | 10.6 | 65.0 | 15.4 | 0.191 | 0.045 | 0.009 |
| 3 | 51 | 73.1 | 14.3 | 63.5 | 21.7 | 0.213 | 0.043 | 0.009 |
| 4 | 47 | 83.7 | 17.8 | 75.4 | 23.1 | 0.212 | 0.044 | 0.008 |
| 5 | 70 | 74.3 | 9.8 | 57.6 | 15.6 | 0.201 | 0.042 | 0.009 |
| = 284 | ||||||||
| Mean ± SD/SEM | 75.5 ± 4.8/2.1 | 13.0 ±3.2/1.4 | 64.8 ± 6.6/2.9 | 19.0 ± 3.5/1.6 | 0.201 ± 0.010/0.005 | 0.042 ± 0.003/0.001 | 0.009 ± 0.001/0.000 | |
| FZP-Withdrawn | ||||||||
| 1 | 55 | 67.3 | 7.5 | 41.8 | 14.6 | 0.191 | 0.037 | 0.007 |
| 2 | 72 | 73.6 | 13.2 | 59.7 | 20.9 | 0.198 | 0.049 | 0.010 |
| 3 | 49 | 77.6 | 10.0 | 65.3 | 14.4 | 0.219 | 0.044 | 0.010 |
| 4 | 48 | 64.6 | 7.9 | 45.8 | 15.0 | 0.219 | 0.039 | 0.009 |
| 5 | 57 | 77.2 | 14.2 | 64.9 | 21.8 | 0.192 | 0.041 | 0.008 |
| = 281 | ||||||||
| Mean ± SD/SEM | 72.1 ± 5.9/2.6 | 10.7 ±3.3/1.5 | 55.5 ± 11.0/4.9 | 17.3 ± 3.7/1.6 | 0.204 ± 0.014/0.006 | 0.042 ± 0.005/0.002 | 0.009 ± 0.001/0.000 | |
| p Value | 0.339 | 0.293 | 0.143 | 0.485 | 0.767 | 0.936 | ||