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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Cancer Res. 2009 Dec 22;70(1):78–88. doi: 10.1158/0008-5472.CAN-09-2747

Figure 2. Tumors arising in the same mouse possessed different levels of XBP1-luc activity.

Figure 2

(A) Left panel: Quantitation of bioluminescence activity in tumor bearing Tag-Luc and Tag mice. Middle panel: In vitro luciferase activity of mammary tumors. Mammary tumors were excised from the indicated mice, diced into fine fragments and lysed. Luciferase activity of each tumor was assessed and normalized to the luciferase activity of the autologous liver in the same mouse. Data are represented as the mean ± SEM. Right panel: Luciferase activity in vitro correlated with bioluminescence activity in vivo. Transgenic mice were imaged and bioluminescence activity quantitated. In vitro luciferase activity of each corresponding tumor was then assessed and compared to its in vivo bioluminescence. (B) Left panel: Mammary tumors in Tag-Luc mice had different levels of bioluminescence. Right panel: Heterogeneity of bioluminescence in vivo correlated with luciferase activity in vitro. Outlined tumors were excised, finely minced, lysed and analyzed for luciferase activity. (C) In vitro analysis of luciferase activity in multiple tumors from individual mice showed heterogeneity of luciferase activity. Chart legend in (C) is the same as in (B, right panel). * represent p <0.05 by Student’s t test.