Figure 7.

GI-LTP structural plasticity requires actin polymerization and CP-AMPAR-mediated activation of Rac. A, Group data of spine head area (n = 50–75 spines per neuron; 5 neurons per coverslip) for controls and neurons subjected to GI-LTP in absence or presence of 10 μm latrunculin A. Error bars indicate SEM (n = 5 coverslips/condition). **p < 0.01 by Student's t test. B, Top, Representative Western blot of activated GTP-bound Rac, determined by affinity pulldown using GST-tagged CRIB domain of PAK1, and total Rac for conditions indicated. Bottom, Group data plotted as the ratio of GTP-Rac to total Rac for each condition normalized to the mean control. Error bars indicate SEM (n = 4–6 independent experiments per condition). *p < 0.05 by Student's t test.