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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Clin Cancer Res. 2010 Jan 12;16(2):461–473. doi: 10.1158/1078-0432.CCR-09-1983

Figure 4. Glioma associated cancer-initiating cells trigger T cell apoptosis.

Figure 4

A. After culturing with the glioma associated cancer-initiating cells supernatants, T cells were stimulated with anti-CD3/CD28 and stained with 7-AAD and Annexin V. Compared to medium alone (control), the glioma associated cancer-initiating cells enhanced T cell apoptosis. B. The glioma associated cancer-initiating cells induce T cell apoptosis through cell-to-cell contact. The glioma associated cancer-initiating cells were co-cultured with autologous PBMCs at a 1:10 ratio, and an apoptotic assay was performed after 3 days of culture. C. B7-H1 blockade in cell-to-cell contacting context reduces T cell apoptosis. Both apoptotic and pre-apoptotic T cell percentages decreased when addition of B7-H1 neutralizing antibody was added to culture conditions compared to the isotype control. One representative FACS plot is shown. Similar results were obtained for glioma associated cancer-initiating cells and autologous PBMCs from two other patients. D. Galectin-3 can induce T cell apoptosis in a dose dependent manner within physiological ranges produced by cancer-initiating cells supernatants.