Table 1. Binding parameters for the c-Cbl:peptide interactions.
Peptide | Sequence | kon | koff | Kd | χ2 |
(M −1s−1) | (s−1) | (µM) | |||
pSpry2 | IRNTNEpYTEGPT | 3.7×105 | 0.139 | 0.31 | 4.85 |
ppSpry2 | IRNTNEpYpTEGPT | 7.7×104 | 0.295 | 3.83 | 6.68 |
pEGFR | DSFLQRpYSSDPT | 2.8×105 | 0.279 | 1.00 | 0.68 |
ppEGFR | DSFLQRpYpSSDPT | 6.5×103 | 0.167 | 25.8 | 0.09 |
tpEGFR | DSFLQRpYpSpSDPT | 687 | 0.022 | 31.5 | 3.36 |
χ2 is the statistical error between the experimental and theoretical models. χ2 is defined as Σ (Rf-Rx)2/(n-p), where Rf is the fitted value at a given point, Rx is the experimental value at the same point, n is the number of data points, and p is the number of fitted parameters.