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. 2010 Sep 22;5(9):e12909. doi: 10.1371/journal.pone.0012909

Figure 1. Ctgf KO and Ctgf Lo-Hi allele.

Figure 1

A) Ctgf KO allele. Line 1 is the endogenous Ctgf locus. Line 2 is the Ctgf KO allele construct. Line 3 is the result following homologous recombination where exons 3–5 of the Ctgf gene were replaced with a neo cassette to generate the Ctgf KO allele. Black boxes indicate coding exons and a white box indicates the endogenous 3′UTR. B) Ctgf Lo-Hi allele. Line 1 is the endogenous Ctgf locus. The Ctgf Lo-Hi allele was generated by replacement of the endogenous Ctgf-3′UTR with a construct containing the c-fos-3′UTR and a Neo cassette flanked by loxP sites, followed by the bGH-3′UTR. In line 2, the modified Ctgf gene uses the less stable c-Fos-3′UTR which reduces gene expression (Ctgf Lo allele). In the bottom line, following Cre-mediated recombination, the c-Fos-3′UTR and Neo cassette are excised and the Ctgf gene uses the more stable bGH-3′UTR which increases gene expression (Ctgf Hi allele). TAA indicates a stop codon Restrcition sites are abbreviated B is BamH1, Bc is Bcl1, Nhe is Nhe1, Nsi is Nsi1, and Bg is Bgl2.