Skip to main content
. 2010 Sep 22;5(9):e12909. doi: 10.1371/journal.pone.0012909

Figure 2. Ctgf mRNA levels by quantitative RT-PCR.

Figure 2

A) Ctgf mRNA expression from tail samples of 10 day pups from a cross of Ctgf Lo/+ parents. All mice are siblings. The mean of Ctgf +/+ (wild type) expression is set to 100% expression LoLo: n = 5, Lo/+: n = 25, and +/+: n = 21. B) Ctgf mRNA expression from tissues of five month old mice. Ctgf Lo/+ mice are the control with Lo/+ expression set to 85% based on results from Figure 2A. For each organ Lo/+: n = 14 and Lo/KO: n = 8. C) Ctgf mRNA expression from whole mouse embryos at day points E11.5, normalized to Lo/+ the same as Figure 2B. Lo/+: n = 8 and Hi/+: n = 13 D) Ctgf mRNA expression from tissues of 10 month old mouse tissues normalized to a combined control of animals of the genotypes +/+;Cre-, Lo/+;Cre-, and +/+;Cre+. E) Neo gene copy number in embryos at day E11.5. Copy number is in arbitrary units and normalized to Lo/+ = 100%. Lo/+: n = 18 and Hi/+: n = 28. For all graphs the Lo/+ animals are heterozygous for the Ctgf Lo allele and do not have a Cre allele. The Hi/+ animals are heterozygous for the Ctgf Hi allele and have one EIIa-Cre allele.