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. 2010 Sep 22;5(9):e12878. doi: 10.1371/journal.pone.0012878

Figure 2. EGCG does not compete with ATP binding of RIG-I.

Figure 2

(A) EGCG does not compete with ATP binding of RIG-I. Effect of ATP concentration on EGCG inhibition of recombinant full length RIG-I ATPase activity. Different amounts of ATP were added to the shR9 dependent ATPase activity in the presence of EGCG (1 µM) or DMSO. The data are shown as a mean +/− standard deviation. (B) Effect of EGCG on ATPase activity deficient mutant K270A in cell-based reporter assay. Signaling by WT and K270A mutant was analyzed using triphosphorylated RNA shR9 and dsRNA mimic, poly(I∶C) in the presence and absence of EGCG (2 µM). K270A was incapable of signaling with shR9 but could induce signaling with poly(I∶C). Signaling of WT RIG-I with shR9 was set as 100%. Assay was performed using IFN-β luciferase as reporter plasmid and the data are shown as a mean +/− standard deviation.