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. 2010 Sep 22;5(9):e12872. doi: 10.1371/journal.pone.0012872

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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LC (0.4 µg, closed circle) or LC+Hn (1.8 µg, closed triangle) and 1 mM peptide substrate in a 30 µl of reaction mixture containing ZnCl₂ and DTT was incubated at 37°C. At the indicated times, products in two vials was analyzed by HPLC as described in the EXPERIMENTAL PROCEDURES.