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. 2010 Sep 22;5(9):e12872. doi: 10.1371/journal.pone.0012872

Figure 8. Reducing SDS-PAGE of LC variants before (C), and after 48-h incubation at 22°C in the absence (−) and presence (+) of 5 mM DTT.

Figure 8

Samples (0.15 mg/ml) were heated at >95°C for 5 min in the SDS-load buffer containing 5% β-mercaptoethanol before electrophoresis. Molecular mass in kDa of the marker proteins in the right lane are shown at right.