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. 2010 Sep 22;5(9):e12928. doi: 10.1371/journal.pone.0012928

Figure 2. Myostatin is attenuated in spleens of HFDIO-resistant mice fed high fat.

Figure 2

Molecular regulation of myostatin (MSTN) mRNA levels using quantitative real-time PCR. Myostatin mRNA levels in muscle from C57BL/6 (a) and SWR/J (c) and spleen from C57BL/6 (b) and SWR/J (d). Muscle and spleen samples were taken at 3, 6, 9, and 12 wk on the dietary treatment. (e) RT-PCR analysis of myostatin mRNA expression in different tissues for verification. Mar) 100 bp ladder, Spl) whole spleen, Leu) isolated leukocytes (CD3/CD28), mus) pooled skeletal muscle, Adi) adipose tissue, and ntc) no template control. * demonstrates between dietary treatment differences at a given time, P<0.05.