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. 2010 Sep 22;5(9):e12928. doi: 10.1371/journal.pone.0012928

Figure 4. Precursor myostatin expression is higher in HFDIO-susceptible mice.

Figure 4

Representative Western ligand blots demonstrating total muscle (a) and spleen (b) myostatin prodomain immunoreactive peptides (MPIPs). In muscle tissue, MPIPs were detected at 37 and 50 kDa, representing the prodomain of myostatin and the precursor protein, respectively. In spleen tissue, MPIPs were detected at 16 and 32 kDa, representing processed prodomain peptides and potentially further processed peptides, respectively. a.) Representative muscle protein samples from SWR/J and C57BL/J high-fat (HF) and control [7] fed mice. b.) Representative spleen protein samples. Lane 1: molecular weight marker, 2,3,5,7: C57BL/6 spleen, 4,6: SWR spleen, 8: -control, SWR adipose tissue protein. c, d. Relative quantification of arbitrary densitometry values from C57BL/6 (B6) and SWR mice fed control or high-fat diets for 12 weeks. No differences were detected at 9 or 12 wks for any MPIP or for muscle 37 kDa, spleen 16 kDa MPIPs, data not shown. Differences marked with lines were considered significantly different at P<0.05.