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. Author manuscript; available in PMC: 2011 Sep 17.
Published in final edited form as: Cell. 2010 Sep 17;142(6):930–942. doi: 10.1016/j.cell.2010.08.030

Figure 1. Upregulation of DNA demethylase components, and hypomethylated genes in apc mutant embryos and FAP adenomas.

Figure 1

(A-D) Whole mount in situ staining for aid (A), apobec2a (A), apobec2b (A), mbd4 (B), tdg (B), gadd45α (C), gadd45αlike(C), gadd45β (C), gadd45γ (C) and dnmt1 (D) in apc mutants (apcmcr) and siblings (apcwt) zebrafish embryos at 72hpf. (E) MeDIP-qPCR for genes shown (selected from genome-wide MeDIP-ChIP microarray analysis) in apcmcr and apcwt (72hpf) which are either uninjected or injected with aaa Mo (combination of aid, apobec2a and apobec2b morpholinos; 0.5ng each) or mbd4 and tdg morpholinos together (1ng each) or V5-Dnmt1 expressing plasmid (1pg, an amount that rescues Dnmt1 morphants with 97% knockdown of Dnmt1 levels). Dnmt1 protein levels in Figure S1C. (F) MeDIP-qPCR for genes shown (selected from genome-wide MeDIP-ChIP microarray analysis) in human adenomas (A) and matching uninvolved tissues (U) from FAP patients. P1-P10 refers to ten different patients. In both E and F, the Y-axis shows values for each promoter region normalized to a negative control region lacking CpGs, and then normalized to the values from wild type or uninvolved, valued at 1. Error bars indicate +/− SD. See also Figure S1.