Figure 5. Lef1 and Groucho2 suppress rdh1l in apc^mcr zebrafish.

(A) Whole mount in situ staining for lef1, groucho2, tcf4 or groucho3 in apc^wt and apc^mcr embryos at 72hpf. Red arrow points to the intestine. (B) Whole mount in situ staining for fabp2, trypsin, rdh1l and irbp in apc^mcr/control Mo, apc^mcr /lef1 Mo and apc^mcr /groucho2 Mo embryos at 72hpf. Groucho2 mo efficiency in Figure S5G. (C-D) ChIP for Lef1 on rdh1l promoter in apc^wt and apc^mcr embryos. (C) Schematic showing the Lef1 sites on the rdh1l promoter and location of the primer set P1 (containing Lef1 sites) and P2 (without Lef1 sites). (D) Graph showing fold enrichment of Lef1 occupancy at P1/P2 on rdh1l promoter in apc^mcr embryos compared to apc^wt at 72hpf. Lef1 values were normalized to ones obtained using a non-specific antibody and then expressed as fold enrichment compared to apc^wt. Error bars indicate +/− SD. (E) Whole mount in situ staining for fabp2 and gata6 in 72hpf embryos injected with full length lef1 or dominant negative (deficient in β-catenin binding) lef1 mRNA alone or with groucho2 mRNA. See also Figure S5.