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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: J Biophotonics. 2010 Oct;3(10-11):706–717. doi: 10.1002/jbio.201000058

Figure 8.

Figure 8

Fluorescence intensity trajectory from a conventional CdSe/ZnS core/shell NQD system [Invitrogen 605 ITK amino (PEG)] diffusing in 80/20 (v/v) glycerol-water (a)-bottom. Data are binned in 1 ms intervals. Three-dimensional trajectory of this nanoparticle recorded in 5 ms intervals is shown in (a)-top left. The dimensions of the cube containing the particle are 3×3×3 μm3. The rainbow color scheme is correlated with that used for the fluorescence intensity trajectory to show the time dependence. The mean-squared displacement (MSD) of the nanoparticle as a function of measurement time interval (Δt) is shown in (a)-top right. In the case of pure diffusive motion in three dimensions (as is the case here), MSD and Δt are linearly related with a slope of six times the diffusion coefficient (D). Fluorescence intensity trajectory for CdSe/19CdS g-NQDs diffusing in 60/40 (v/v) glycerol-water is shown in (b)-bottom. A g-NQD trajectory (cube dimensions: 11×11×11 μm3) and g-NQD MSD are shown in (b)-top left and top-right, respectively.