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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: J Immunol. 2010 Sep 8;185(7):4344–4353. doi: 10.4049/jimmunol.0904191

Figure 7. Incubation of N. gonorrhoeae strain 252 with fH enhances bacterial binding to CHO/CR3 cells.

Figure 7

Binding of GFP-expressing unsialylated or sialylated gonococci (strain 252) to CHO/NEO (control) cells (A) or CHO/CR3 cells (B) in the presence (grey shaded histograms) or absence (histograms depicted by solid lines) of fH (concentration, 67 nM); determined by flow cytometry. Bacteria were added to CHO cells at an MOI of 100:1. (C) Binding experiments performed using CHO/CR3 cells and organisms coated with CFHR1 and (D) using FHL-1. Events were gated to include CHO cells only (with or without bound bacteria and excluding free bacteria). GFP fluorescence (on the x-axis) reflects the bacteria associated with CHO cells, the y-axis the number of events. Numbers in the histogram represent the GFP median fluorescence of the CHO/NEO and CHO/CR3 cell population. One representative experiment is shown of three independently performed experiments.