Table 2.

Thickness and roughness of multiple porous silica layers measured with AFM (Digital Instruments Series IV). Layer parameters in bolded cells were found to result in the most successful protein transfer.

Porosity	Thickness	Pore Size	Contact Angle	Protein Layer Thickness	Protein Layer Roughness
63%	547 nm	6.3 nm	11.1 ± 0.2°	3 ± 2 nm	3 ± 1 nm
39%	611 nm	4.0 nm	13.3 ± 0.7°	8 ± 5 nm	3 ± 2 nm
57%	673 nm	4.0 nm	12.8 ± 0.8°	8 ± 4 nm	5 ± 2 nm
32%	561 nm	2.0 nm	23 ± 2°	3 ± 2 nm	1.5 ± 0.4 nm
57%	30 nm	4.0 nm	30 ± 3°	5 ± 1 nm	1.1 ± 0.6 nm
57%	96 nm	4.0 nm	13 ± 6°	3 ± 1 nm	3.4 ± 0.4 nm
53%	223 nm	4.0 nm	15 ± 2°	5 ± 3 nm	5.0 ± 0.5 nm