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. 2010 Jul 23;29(17):2966–2978. doi: 10.1038/emboj.2010.171

Figure 2.

Figure 2

Claspin mRNA is regulated by IKKβ and the NF-κB family of transcription factors. (A) Semi-quantitative and quantitative RT–PCR analysis of Claspin mRNA prepared from U2OS cells depleted of IKKα, IKKβ or Claspin by siRNA. (B) U2OS cells were depleted of the individual NF-κB family members; RelA, RelB, c-Rel, p105/p50 or p100/p52, using siRNA and analysed by immunoblot using the indicated antibodies. (C) Semi-quantitative RT–PCR analysis of Claspin, RelA, RelB, c-Rel, p105, p100 and actin mRNA from cells treated as in (A). (D) Quantitative RT–PCR analysis of Claspin mRNA in cells depleted of the individual NF-κB subunits as in (A). ANOVA t-tests were performed on the means, and the P-values were calculated. *P⩽0.05, **P⩽0.01, ***P⩽0.001.