Table 3. Early arrival of GFP-Myo5 to the endocytic sites in the cmd1-226 is accompanied by early recruitment of Abp1-RFP.

Strain	CMD1 GFP-MYO5 Abp1-RFP (SCMIG1063 pGFP-MYO5)			cmd1-226 GFP-MYO5 Abp1-RFP (SCMIG1064 pGFP-MYO5)			P
Parameter	Avg	s.d.	n	Avg	s.d.	n
Ls Abp1-RFP	13.5	2.5	20	20.7a	5.2a	20	4 × 10−4
Ls GFP-Myo5	11.7	1.9	20	24.3a	5.2a	20	2 × 10−6
ta GFP-Myo5	−0.05	2.8	20	−0.08a	3.4a	20	0.6436
td GFP-Myo5	−1.9	2.4	20	3.5a	4.25a	20	4 × 10−3
Lifespans (Ls) of 20 correlative Abp1-RFP and GFP-Myo5 patches and arrival (ta) and departure times (td) of GFP-Myo5 relative to Abp1-RFP, analysed in the indicated strains (see Materials and methods and Figure 7). The average (avg) and standard deviation (s.d.) are indicated in seconds. ‘n' represents the number of pairs of cortical patches analysed for the indicated yeast strains. ‘P' represents the P-value of the Student's t-test. P-values under 0.001 indicate statistically significant differences.
aSubpopulation associated with GFP-Myo5 cortical patches with lifespans longer than 16 s.