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. 2010 Jul 30;29(18):3108–3117. doi: 10.1038/emboj.2010.180

Figure 3.

Figure 3

The effect of the A2503 and A2062 mutations upon induction of the ermC-based reporter. (A) The structure of the ermC-based pZα101t reporter. A portion of the lacZ gene encoding the β-galactosidase α-peptide is fused to the first two codons of ermC. Ribosome stalling at the ermCL ORF induces expression of the ermC-lacZα fusion. (B) Drug-diffusion induction of the pZα101t reporter in E. coli cells expressing wild-type or mutant ribosomes. The plates contain a lawn of SQK15/pZα101t cells grown on the surface of LB agar plates supplemented with ampicillin, tetracycline, IPTG, and X-gal. Expression of the reporter is induced by diffusion of erythromycin from the paper disk containing 0.5 mg of the drug. (C) β-Galactosidase activity (nmol/min/mg) in the wild-type and mutant SQK15 cells containing the pZα101t reporter.