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. 2010 Aug 10;29(18):3054–3067. doi: 10.1038/emboj.2010.187

Figure 4.

Figure 4

The ‘lipid-uncoupled' S619L variant, but not the ‘lipid-sensitized' A618T variant, forms higher-ordered oligomers at elevated temperatures. Wild-type and selected mutants (1 μM) were pre-incubated at 22 or 37°C for 10 min, centrifuged at high speed, and then analysed by size exclusion chromatography (SEC). (A) SEC profile of the differently treated wild-type and A618T variants. (B) Analysis of the S619L mutant shows oligomerization after incubation at 37°C (a 22°C wild-type profile is re-plotted for reference).