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. 2010 Aug 6;29(18):3130–3139. doi: 10.1038/emboj.2010.188

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Copyright © 2010, European Molecular Biology Organization

This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial Share Alike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission.

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CHD4 is recruited to sites of laser-induced DNA damage within the NuRD complex. (A) Immunodetection of CHD4 and γH2AX (damage sites) at the indicated times after laser micro-irradiation in U2OS cells. (B) Recruitment of NuRD complex subunits to sites of laser-induced DNA damage (labelled by γH2AX) 5 min after micro-irradiation in U2OS cells. (C, D) Immunodetection of HDAC1 and CHD4 5 min after laser micro-irradiation in U2OS cells treated with the indicated siRNAs (siLuci: control). Cells were pre-treated with ATM inhibitor to facilitate detection of HDAC1 and CHD4 lines. Lower panels show siRNA efficiency. In laser micro-irradiation experiments, detergent pre-extraction was performed before fixation of the cells for immunostaining. Scale bars, 10 μm.