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Effects of PHR, HC, EGF, and E2 on Focus Formation by MCF-10A Cells Cultured for 13 Days. MCF-10A cells, plated at 5 × 105 cells/well, were grown in 6-well tissue culture plates under differing culture and treatment (once a week) conditions and photographed at low magnification (phase contrast; 4×) to enable visual indication of the number of transformed foci after13 days in culture. Photographs are representatives of triplicate samples from one experiment.
