Figure 4.
Effects of 1 nM 2-Fl-E2 Treatment on Focus Formation by MCF-10A Cells Grown in Continuous Culture for 5 Weeks. MCF-10A cells, plated at 5 × 105 cells/well, were grown in 6-well TC plates in -PHR/-HC/-EGF medium and treated with 1 nM 2-Fl-E2 once a week. Two different areas of one well were photographed (phase contrast; 10×) in order to show variations in cell morphology and focus formation within the same treatment group at 5 weeks in continuous culture. Photographs are representatives of triplicate samples from one experiment.