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Matrix metalloproteinase-25 (MMP-25) mRNA and protein are expressed in the developing mouse palate at all stages. (A) Quantitative real-time PCR indicates a significant down-regulation in MMP-25 mRNA levels at E15.5 compared to E12.5 and E13.5 (p < 0.05; n = 4 for all stages). Error bars indicate standard error of the mean. (B) Western blot analysis displays a reduction in MMP-25 protein levels at E15.5 relative to E12.5 and E13.5. GAPDH was used as a loading control.
