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Representative hematoxylin and eosin staining of embryonic day (E) 13.0 in vitro cultured palates after 72 h. (A) Palate shelves incubated for 72 h with growth medium fused normally. (B) Palate shelves grown with 500 nM scrambled control siRNA fused normally. (C) Palate shelves grown with 500 nM matrix metalloproteinease-25-specific siRNA did not fuse normally and contained considerable medial edge epithelium compared to the control groups.
